Studies on Mucosal Immunity Induced by Transmissible Gastroenteritis Virus Nucleocapsid Protein Recombinant Lactobacillu
Mucosal immunity plays an important role in protecting pigs against transmissible gastroenteritis virus (TGEV) infection. To elicit mucosal immune response against TGEV, we developed a surface antigen display system using the poly-γ-glutamate synthetase A (pgsA) protein of Bacillus subtilis as an anchoring matrix to express recombinant fusion proteins of pgsA and nucleocapsid protein of TGEV in Lactobacillus casei. Surface location of fusion protein was verified by ELISA and indirect immunofluorescence test. Oral and intranasal inoculations of pregnant sow and mice with recombinant L. casei resulted in high levels of serum immunoglobulin G (IgG) and secretory immunogiobulin A (sIgA) against recombinant N protein as demonstrated by ELISA. More importantly, the level of specific slgA in colostrum significantly increased compared with that of IgG. The serum lgG levels of the piglets increased after suckling coiostrum produced by sows was previously inoculated with recombinant L. casei. These results indicate that immunization with recombinant L. casei expressing TGEV N protein on its surface elicited high levels of specific slgA and circulating lgG against TGEV N protein.
作 者： WANG Gui-hua HOU Xi-lin YU Li-yun LIU Jian-kui WEI Chun-hua 作者单位： WANG Gui-hua,YU Li-yun,LIU Jian-kui(College of Life Science and Technology, Heilongjiang August First Land Reclamation University, Daqing 163319, P.R.China)HOU Xi-lin,WEI Chun-hua(College of Animal Science and Technology, Heilongjiang August First Land Reclamation University, Daqing 163319, P.R.China)
刊 名： 中国农业科学（英文版） 英文刊名： AGRICULTURAL SCIENCES IN CHINA 年，卷(期)： 2009 8(2) 分类号： S1 关键词： transmissible gastroenteritis viral lactic acid bacteria N protein