The effects of Fumaderm(R) on immunological function and cytokines in a rat model of adjuvant-induced arthritis
Objective To study the therapeutic effect of Fumaderm? in Freund' complete adjuvant-induced arthritis (AIA) in Spraque-Dawley rats. Methods Adjuvant-induced arthritis (AIA) was established by intradermal injection of 0.1 mL of Freund' complete adjuvant (CFA) in the palmar surface of the right hindpaw and Fumaderm? was delivered by oral gavage for 28 days. After CFA injection, the edema of the hindpaw was determined every two days. On 28 days after CFA injection, the lymphocyte subsets of peripheral blood and the cytokines were determined by flow cytometry, meanwhile the histopathological examination of ankle-joints of the animals was performed. Results Fumaderm? had a significant therapeutic effect on AIA. The hindpaw swelling was reduced significantly in a dose-dependent manner. The ratio of peripheral blood T lymphocytes was improved obviously. Multiparameter cytokine analysis from peripheral blood CD4+ T cells showed a decrease of proinflammatory cytokines and an increase of anti-inflammatory cytokines in Fumaderm? treated animals. A strongly reduced inflammatory response in the joint synovium was observed. Conclusion Fumaderm? has potential anti-inflammatory effects on AIA rats. Further investigation is needed to elucidate the molecular mechanism involved in the clinical effect observed in the AIA model.
作 者： Dian-Zeng Zhang Hong-Ying Wang Feng Zhao Feng Wu 作者单位： Dian-Zeng Zhang,Feng Zhao,Feng Wu(The Center for Biomedical Research, Key Laboratory of Environment & Disease Related to Genes, Ministry of Education of China, Medical School of Xi'an Jiaotong University, Xi'an 710061)Hong-Ying Wang(Department of Pharmaceutical Science, Medical School of Xi'an Jiaotong University, Xi'an 710061, China)
刊 名： 西安交通大学学报（英文版） 英文刊名： ACADEMIC JOURNAL OF XI'AN JIAOTONG UNIVERSITY(ENGLISH EDITION) 年，卷(期)： 2010 22(1) 分类号： U4 关键词： fumaric acid esters adjuvant-induced arthritis cytokine immunomodulator