Cloning and characterization of a new actin gene from Oryza sativa L.
Using Rho family member osRACD as bait, a new member of actin gene family -Act was isolated from Oryza sativa by yeast two-hybrid system. The full-length cDNA was cloned with 5' RACE technology, which contains an open reading frame of 1134 bp with a predicted protein of 377 amino acids. Sequence alignment revealed 96% to 81.8% identities with some known actin proteins in plants. The method of bioinformatics was used to analyze the protein modification sites, structure and evolution of the gene. Southern blot analysis showed that Act is a single-copy gene in the genome. The result of RT-PCR showed it is ubiquitously expressed in root, shoot, callus and panicle in a temporal fashion. The relationship between Rho family and actin family in evolution and function was also studied.
作 者： LIANG Weihong TANG Chaorong WU Naihu 作者单位： Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100080, China 刊 名： 自然科学进展(英文版) SCI 英文刊名： PROGRESS IN NATURAL SCIENCE 年，卷(期)： 2004 14(10) 分类号： Q7 关键词： rice panicle yeast two-hybrid actin Rho gene clone