Transferring gfp gene with ion implantation and transient expression of gfp in liliaceous pollen cells
Liliaceous pollen cells were implanted by 4.0 MeV C2+ ion beam or by 25.0 keV N+ ion beam. Laser confocal scanning microscopy (LCSM) of the implanted intact samples showed that parts of the implanted pollen cells could be stained by propidium iodide (PI). This indicated that energetic ion beam could directly act on cells beneath the pollen coats and made channels for entry of the molecules from outside of the cells. LCSM analysis of green fluorescent protein (GFP) showed that energetic ion beam could mediate transient expression of gfp in treated pollen cells. Compared with 25.0 keV N+ ion beam, implantation of 4.0 MeV C2+ ion beam greatly improved gene transfer efficiency in pollen cells.
作 者： YUAN Shibin CHEN Qizhong WANG Yugang Zhao Weijiang XU An YANG Gen Wang Wenxian WU Lijun 作者单位： YUAN Shibin,CHEN Qizhong,WANG Yugang,Zhao Weijiang(Institute of Heavy Ion Physics, Peking University, Beijing 100871, China)XU An,YANG Gen,Wang Wenxian,WU Lijun(Institute of Plasma Physics, Chinese Academy of Sciences, Hefei 230031, China)
刊 名： 自然科学进展(英文版) SCI 英文刊名： PROGRESS IN NATURAL SCIENCE 年，卷(期)： 2004 14(11) 分类号： Q3 关键词： MeV ion implantation radiation damage pollen gfp transient expression