In silico Analysis of the Potential Infection Mechanisms of Magnaporthe grisea from Horizontal Gene Transfer Hypothesis
Horizontal gene transfer(HGT)has long been considered as a principal force for an organism to gain novel genes in genome evolution. Homology search, phylogenetic analysis and nucleotide composition analysis are three major objective approaches to arguably determine the occurrence and directionality of HGT. Here, 21 genes that possess the potential to horizontal transfer were acquired from the whole genome of Magnaporthe grisea according to annotation, among which three can-didate genes(corresponding protein accession numbers are EAA55123, EAA47200 and EAA52136)were selected for further analysis. According to BLAST homology results, we subsequently conducted phylogenetic analysis of the three candidate HGT genes. Moreover, nucleotide composition analysis was conducted to further validate these HGTs. In addition, the functions of the three candidate genes were searched in COG database. Consequently, we conclude that the gene encoding protein EAA55123 is transferred from Clostridium perfringens. Another HGT event is between EAA52136 and a certain metazoan's corresponding gene, but the direction remains uncertain. Yet, EAA47200 is not a transferred gene.
作 者： Chunyang Li Ying Wang Hao Peng Hejiao Bian Mingwei Min Longfei Chen Qian Liu Jinku Bao 作者单位： Chunyang Li,Hao Peng,Hejiao Bian,Mingwei Min,Longfei Chen,Qian Liu,Jinku Bao(School of Life Sciences, Sichuan University, Chengdu 610064, China)Ying Wang(China National Center for Biotech-nology Development, Beijing 100081, China)
刊 名： 基因组蛋白质组与生物信息学报（英文版） 英文刊名： GENOMICS、PROTEOMICS & BIOINFORMATICS 年，卷(期)： 2009 7(3) 分类号： Q5 关键词： Magnaporthe grisea infection mechanism horizontal gene transfer homology phy-logenetic analysis nucleotide composition