Ohmefentanyl stereoisomers induce changes of CREB phosphorylation in hippocampus of mice in conditioned place preference
The present study was designed to determine the changes of phosphorylation of cAMP- response ele-ment binding protein (CREB) in hippocampus induced by ohmefentanyl stereoisomers (F9202 and F9204)in conditioned place preference (CPP) paradigm. The results showed that mice receiving F9202 and F9204displayed obvious CPP. They could all significantly stimulate CREB phosphorylation and maintained for along time without affecting total CREB protein levels. The effect of F9204 was similar to morphine whicheffect was more potent and longer than F9202. We also examined the effects of ketamine, a noncompetitiveN-mthyl-D-aspartate receptor (NR) antagonist, on morphine-, F9202- and F9204- induced CPP and phos-phorylation of CREB in hippocampus. Ketamine could suppress not only the place preference but also thephosphorylation of CREB produced by morphine, F9202 and F9204. These findings suggest that alterationsin the phosphorylation of CREB be relevant to opiates signaling and the development of opiates dependence.NR antagonists may interfere with opiates dependence and may have potential therapeutic implications.
作 者： Can GAO LI WEI CHEN Jie CHEN XUE JUN XU ZHI QIANG CHI 作者单位： Can GAO(Second Department of Pharmacology, Shanghai Institute of Materia Medica, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China;Research Center for Biochemistry and Molecular Biology, Xuzhou Medical College, Xuzho)LI WEI CHEN,Jie CHEN,XUE JUN XU,ZHI QIANG CHI(Second Department of Pharmacology, Shanghai Institute of Materia Medica, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China)
刊 名： 细胞研究(英文版) ISTIC SCI 英文刊名： CELL RESEARCH 年，卷(期)： 2003 13(1) 分类号： Q2 关键词： cAMP-response element binding protein (CREB) ohmefentanyl stereo isomers opiates hippocampus conditioned place preference (CPP)