Identification of ABA-responsive genes in rice shoots via Cdna macroarray
Phytohormone abscisic acid (ABA) was critical for many plant growth and developmental processesincluding seed maturation, germination and response to environmental factors. With the purpose to detectthe possible ABA related signal transduction pathways, we tried to isolate ABA-regulated genes throughcDNA macroarray technology using ABA-treated rice seedling as materials (under treatment for 2, 4, 8 and12 h). Of 6144 cDNA clones tested, 37 differential clones showing induction or suppression for at least onetime, were isolated. Of them 30 and 7 were up- or down-regulated respectively. Sequence analyses revealedthat the putative encoded proteins were involved in different possible processes, including transcription,metabolism and resistance, photosynthesis, signal transduction, and seed maturation. 6 cDNA clones werefound to encode proteins with unknown functions. Regulation by ABA of 7 selected clones relating to signaltransduction or metabolism was confirmed by reverse transcription PCR. In addition, some clones werefurther shown to be regulated by other plant growth regulators including auxin and brassinosteroid, which,however, indicated the complicated interactions of plant hormones. Possible signal transduction pathwaysinvolved in ABA were discussed.
作 者： FANG LIN SHOU LING XU WEI MIN NI ZHAo QING CHU ZHI HONG XU HONG WEI XUE 作者单位： National Laboratory of PlantMolecular Genetics,Institute of Plant Physiology and Ecology,Shanghai Institutes for Biological Science(SIBS),Chinese Academy of Sciences;and Partner Group of Max-Planck-Institute of Molecular Plant Physiology(MPI-MP)on"Plant M 刊 名： 细胞研究(英文版) ISTIC SCI 英文刊名： CELL RESEARCH 年，卷(期)： 2003 13(1) 分类号： Q2 关键词： Oryza sativa abscisic acid (ABA) cDNA macroarray responsive genes signaltransduction