Distinct expression profiles of transcriptional coactivators for thyroid hormone receptors during Xenopus laevis metamor
The biological effects of thyroid hormone(T3)are mediated by the thyroid hormone receptor(TR).Amphibian metamorphosis is one of the most dramatic processes that are dependent on T3.T3 regulates a series of orchestrated developmental changes,which ultimately result in the conversion of an aquatic herbivorous tadpole to a terrestrial carnivorous frog.T3 is presumed to bind to TRs,which in turn recruit coactivators,leading to gene activation.The best-studied coactivators belong to the p 160 or SRC family.Members of this family include SRC 1/NCoA- 1,SRC2/TIF2/GRIP 1,and SRC3/pCIP/ACTR/AIB- 1/RAC-3/TRAM- 1.These SRCs interact directly with liganded TR and function as adapter molecules to recruit other coactivators such as p300/CBP.Here,we studied the expression patterns of these coactivators during various stages of development.Amongst the coactivators cloned in Xenopus laevis,SRC3 was found to be dramatically upregulated during natural and T3-induced metamorphosis,and SRC2 and p300 are expressed throughout postembryonic development with little change in their expression levels.These results support the view that these coactivators participate in gene regulation by TR during metamorphosis.
作 者： BINDU D PAUL Yun-Bo SHI 作者单位： Laboratory of Gene Regulation and Development,National Institute of Child Health and Human Development,National Institutes of Health,Bldg.USA. 刊 名： 细胞研究(英文版) ISTIC SCI 英文刊名： CELL RESEARCH 年，卷(期)： 2003 13(6) 分类号： Q2 关键词： transcription coactivators thyroid hormone receptor Xenopus laevis metamorphosis histone acetylation