Effect of nerve regeneration factor on differentiation of PC12 cells and its signaling pathway
The effects of nerve regeneration factor (NRF) on neuronal differentiation of PC12 cells and its signaling pathway are investigated by morphological observation and immunofluorescent cytochemical method, and the activity of ERK1/2 in NRF-treated PC12 cells in absence of serum is also studied by immuno-coprecipitation and Western blot analysis. The MEK1/2-specific inhibitor U0126, the broad-spectrum protein kinase C (PKC) inhibitor G?6983 and tyrosine protein kinase (TPK) inhibitor genistein were used to determine the roles of the activation of ERK1/2 by NRF and the involvement of certain kinds of PKC or TPK receptor in this activation process. The results show that U0126 and G?6983 inhibit the activation of ERK1/2 by NRF to different extents, while genistein has no effect on it, demonstrating that NRF remarkably induces neuronal differentiation of PC12 cells through activating ERK1/2 in a dose-dependent and time-dependent manner.
作 者： DING Fei QIANG Liang LIU Mei GU XingXing Gu Xiaosong 作者单位： The Key Laboratory of Neuroregeneration of Jiangsu Province, Nantong Medical College, Nantong 226001, China 刊 名： 自然科学进展(英文版) SCI 英文刊名： PROGRESS IN NATURAL SCIENCE 年，卷(期)： 2004 14(11) 分类号： Q4 关键词： nerve regeneration factor PC12 cells neuronal differentiation ERK1/2