The transfection of embryonic stem c

The transfection of embryonic stem cells with Tet-on system and its responsiveness to doxycycline

We transiently transfected pTet-on and pTRE2hyg-luciferase into the mouse embryonic stem cells (ESCs) using lipofectamine, and analyzed its inductive effect by adding serial concentrations of doxycycline (DOX). The results showed that in the transfected group, the luciferase activity of the cells was gradually increased along with the increasing concentration of DOX. While in the non-transfected group, the luciferase activity was not detectable even with DOX treatment. This indicated that the ESCs transfected with Tet-on system could response to DOX very well, and the regulation of target gene expression is dose dependent.

作 者： WANG Yan CONG Xiaoqian LIU Deli ZHANG Wenjie CUI Lei LIU Wei CAO Yilin   作者单位： Department of Plastic and Reconstructive Surgery, Shanghai 9th People's Hospital, Shanghai Second Medical University, Shanghai Key Laboratory of Tissue Engineering, Shanghai 200011, China  刊 名： 中国科学C辑（英文版）  SCI 英文刊名： SCIENCE IN CHINA (LIFE SCIENCES)  年，卷(期)： 2005 48(4)  分类号： Q1  关键词： gene expression   embryonic stem cell  

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