Efficient and stable generation of transgenic silkworm, Bombyx mori with the lepidopteran derived transposon piggyBac
The piggyBac transposable element was successfully used for generation of the transgenic silkworm, Bombyx mori. The EGFP was adopted in this study as a screening marker in transgenic vector under the control of an artificial promoter containing Pax-6 binding sites that can drive eye-specific genes expression in various insect species including B. mori and Drosophila. 111 independent transgenic lines were obtained among 700 fertile G0 moths. Most of the transgenic lines contained two or more chromosomal insertions of the EGFP marker, which were stably inherited over more than six generations during the time of this project. PiggyBac-mediated transposition was confirmed by identifying the characteristic TTAA duplication sequence at the insertion sites. The stable and high efficient transmission of a genetic marker into B. mori confirms the usage of this vector-marker system for industrial production and theoretical research.
作 者： DAI Hongjiu XU Guojiang Thomas Jean-luc WANG Zhugang JIANG Rongjing FEI Jian 作者单位： DAI Hongjiu(Graduate School of Chinese Academy of Sciences, Institute of Plant Physiology and Ecology Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China;Shanghai Research Center for Model Organism, Shanghai 201203, China;Model Organism Research Center, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China)XU Guojiang(Shanghai Research Center for Model Organism, Shanghai 201203, China)
Thomas Jean-luc(Unité Nationale Séricicole/INRA 25 Quai J.J.Rousseau 69350 La Mulatiére, France)
WANG Zhugang(Shanghai Research Center for Model Organism, Shanghai 201203, China;Model Organism Division, e-Institutes of Shanghai Universities, Shanghai 200025, China)
JIANG Rongjing(Graduate School of Chinese Academy of Sciences, Institute of Plant Physiology and Ecology Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China)
FEI Jian(Shanghai Research Center for Model Organism, Shanghai 201203, China;Model Organism Research Center, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China;Model Organism Division, e-Institutes of Shanghai Universities, Shanghai 200025, China)
刊 名： 科学通报（英文版） SCI 英文刊名： CHINESE SCIENCE BULLETIN 年，卷(期)： 2005 50(15) 分类号： Q1 关键词： Bombyx mori gene transgene piggyBac screening marker