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One New Method of Nucleic Acid Ampli

时间:2021-12-12 19:44:52 生物医学论文 我要投稿

One New Method of Nucleic Acid Amplification-Loop-mediated Isothermal Amplification of DNA

Loop-mediated isothermal amplification (LAMP) is a novel nucleic acid amplification method, which amplifies DNA with high specificity, sensitivity, rapidity and efficiency under isothermal conditions using a set of four specially designed primers and a Bst DNA polymerase with strand displacement activity. The basic principle, characteristics, development of LAMP and its applications are summarized in this article.

作 者: Xue-en FANG Jian LI Qin CHEN   作者单位: Xue-en FANG,Qin CHEN(School of Life Science, Shanghai University, Shanghai 200444, China)

Jian LI(Shanghai Entry-Exit Inspection and Quarantine Bureau, Shanghai 200135, China) 

刊 名: 中国病毒学  ISTIC 英文刊名: VIROLOGICA SINICA  年,卷(期): 2008 23(3)  分类号: Q7  关键词: Nucleic acid amplification   Loop-mediated isothermal amplification (LAMP)   Application