One New Method of Nucleic Acid Amplification-Loop-mediated Isothermal Amplification of DNA
Loop-mediated isothermal amplification (LAMP) is a novel nucleic acid amplification method, which amplifies DNA with high specificity, sensitivity, rapidity and efficiency under isothermal conditions using a set of four specially designed primers and a Bst DNA polymerase with strand displacement activity. The basic principle, characteristics, development of LAMP and its applications are summarized in this article.
作 者: Xue-en FANG Jian LI Qin CHEN 作者单位: Xue-en FANG,Qin CHEN(School of Life Science, Shanghai University, Shanghai 200444, China)Jian LI(Shanghai Entry-Exit Inspection and Quarantine Bureau, Shanghai 200135, China)
刊 名: 中国病毒学 ISTIC 英文刊名: VIROLOGICA SINICA 年,卷(期): 2008 23(3) 分类号: Q7 关键词: Nucleic acid amplification Loop-mediated isothermal amplification (LAMP) Application