Analysis of Hydrolysis Reaction of N-Phosphoryl Phenylalanine by HPLC-ESI-MS/MS
Hydrolysis procedure of N-phosphoryl phenylalanine (DIPP-Phe) was studied by HPLC-ESI-MS/MS. The results showed that (HO)(i-PrO)P(O)Phe was the main intermediate and the hydrolysis of DIPP-Phe also occurred through a penta-coordinate transition state.
作 者： Shu Xia CAO Jian Chen ZHANG Jun XU Xin Cheng LIAO Yu Fen ZHAO 作者单位： Shu Xia CAO,Jian Chen ZHANG,Jun XU,Xin Cheng LIAO(The Key Laboratory of Chemical Biology,Department of Chemistry,Zhengzhou University,Zhengzhou 450052)Yu Fen ZHAO(The Key Laboratory of Chemical Biology,Department of Chemistry,Zhengzhou University,Zhengzhou 450052;The Key Laboratory for Bioorganic Phosphorus Chemistry and Chemical Biology,Ministry of Education,Department of Chemistry School of Life Science and Engin)
刊 名： 中国化学快报(英文版) ISTIC SCI 英文刊名： CHINESE CHEMICAL LETTERS 年，卷(期)： 2004 15(7) 分类号： 关键词： N-Phosphoryl amino acids hydrolysis reaction HPLC-ESI-MS