DNA Separation by Microchip Electrophoresis Using Copolymers of Poly(vinylpyrrolidone) and Hydroxyethylcellulose
Copolymers of poly(vinylpyrrolidone) (PVP) and hydroxyethylcelhilose (HEC) were synthesized, with PVP to HEC molar ratios of 3:1, 2:1 and 1:1. The copolymers were tested as separation media in DNA fragment separation analysis by microchip electrophoresis (MCE). Separation efficiency over 3.8 ×10~5 for 118 bp has been reached by using the bare channels without the additional polymer coating step. Under optimized separation conditions for longer read length DNA sequencing, the separation ability of the copolymers decreased with decreasing (PVP-co-HEC) molar ratio from 3:1 to 2:1 and 1:1. In comparison with (PVP-co-HEC) 1:1, the copolymer with (PVP-co-HEC) 3:1 ratio showed high separation efficiency. By using a 20 g·L~(-1) copolymer with (PVP-co-HEC) 3:1 ratio, φX174-HaeⅢ digest DNA marker was successfully separated within 3 min.
作 者： LI, Gang GE, Shuli NI, Xiaofang DONG, Shuqing WANG, Qingjiang HE, Pingang FANG, Yuzhi 作者单位： Department of Chemistry, East China Normal University, Shanghai 200062, China 刊 名： 中国化学（英文版） SCI 英文刊名： CHINESE JOURNAL OF CHEMISTRY 年，卷(期)： 2009 27(11) 分类号： O6 关键词： microfluidics DNA separation copolymer