Purification of Pseudomonas sp. Lipa

Purification of Pseudomonas sp. Lipase by Continuous Elution Electrophoresis Based on Pb2+ Precipitation Method

A Pb2+ precipitation method was designed to get rid of the impure proteins in a lipase. The results show that it was a simple way in the primary treatment of the crude samples and about 20% impure proteins were removed in the precipitation step. Further, continuous elution electrophoresis was also applied as a preparative technique for attaining the highly pure lipase. During the continuous elution electrophoresis, the enzyme was eluted as a single peak and 5.7-fold purification was achieved in a yield of 54.3%. The two steps finally yielded an electrophoretically homogeneous enzyme.

作 者： ZHANG Hua-li WANG Zhi LIU Bin WANG Xue-li CAO Shu-gui LI Zheng-qiang   作者单位： Key Laboratory for Molecular Enzymology and Engineering of Ministry of Education, Jilin University, Changchun 130021, P. R. China  刊 名： 高等学校化学研究（英文版）  ISTIC SCI 英文刊名： CHEMICAL RESEARCH IN CHINESE UNIVERSITIES  年，卷(期)： 2005 21(4)  分类号： O6  关键词： Ultrafiltration   Continuous elution electrophoresis   Pb2+ precipitation   Pseudomonas sp. Lipase  

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