Development and Characterization of Microsatellite Markers for the Pacific Abalone (Haliotis discus) via EST Database Mi
The EST database of the Pacific abalone (Haliotis discus) was mined for developing mierosatellite markers. A total of 1476 EST sequences were registered in GenBank when data mining was performed. Fifty sequences (approximately 3.4%) were found to contain one or more mierosatellites. Based on the length and GC content of the flanking regions, duster analysis and BLASTN, 13 microsatellite-containing ESTs were selected for PCR primer design. The results showed that 10 out of 13 primer pairs could amplify seorable PCR products and showed polymorphism. The number of alleles ranged from 2 to 13 and the values of Hoand He varied from 0.1222 to 0.8611 and 0.2449 to 0.9311, respectively. No significant linkage disequilibrium (LD) between any pairs of these loci was found, and 6 of 10 loci conformed to the Hardy-Weinberg equilibrium (HWE). These EST-SSRs are therefore potential tools for studies of intraspecies variation and hybrid identification.
作 者： ZHAN Aibin BAO Zhenmin WANG Mingling CHANG Dan YUAN Jian WANG Xiaolong HU Xiaoli LIANG Chengzhu HU Jingjie 作者单位： ZHAN Aibin,BAO Zhenmin,WANG Mingling,CHANG Dan,YUAN Jian,WANG Xiaolong,HU Xiaoli,HU Jingjie(Laboratory of Marine Genetics and Breeding (MGB), College of Marine Life Sciences, Ocean University of China, Qingdao 266003, P. R. China)LIANG Chengzhu(Shangdong Entry-Exit Inspection and Quarantine Bureau, Qingdao 266002, P. R. China)
刊 名： 中国海洋大学学报（英文版） ISTIC 英文刊名： JOURNAL OF OCEAN UNIVERSITY OF CHINA 年，卷(期)： 2008 7(2) 分类号： P7 关键词： mierosatellite Pacific abalone Haliotis discus EST