Comparative analysis of hydrogen-producing bacteria and its immobilized cells for characteristics of hydrogen production
A strain of hydrogen producing bacteria was immobilized by polyvinyl alcohol-boric acid method,with the addition of a small amount of calcium alginate. The immobilized cells were insensitive to the presence of traces of O2. Moreover, the immobilized cells increased both the evolution rate and the yield of hydrogen production. Batch experiments with a medium containing 10 g/L glucose demonstrated the yields of hydrogen production by the immobilized and free cells were 2.14 mol/mol glucose and 1.69 mol/mol glucose, respectively.In continuous cultures atmedium retention time of 2. 0 h, the yield and the evolution rate of hydrogen producmedium retention time of 6. 0 h, the yield and the evolution rate of hydrogen production by free cells were only 1.75 mol/mol glucose and 362.9ml/(L·h),respectively.
作 者： 王相晶 任南琪 向文胜 王爱杰 林明 郭婉茜 作者单位： 王相晶,任南琪,王爱杰,林明,郭婉茜(School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, China)向文胜(Research Center of Life Science and Biotechnology, Northeast Agricultural University, Harbin 150030, China)
刊 名： 哈尔滨工业大学学报(英文版) EI 英文刊名： JOURNAL OF HARBIN INSTITUTE OF TECHNOLOGY(NEW SERIES) 年，卷(期)： 2003 10(4) 分类号： X703 关键词： hydrogen producing bacteria immobilized cells characteristics of hydrogen production