Effect of Cu2+ concentration on hydrogen fermentation by mixed culture
The results of the study on the effect of Cu2+ concentration on hydrogen production using a glucose solution and mixed microorganisms from a 9.2 L laboratory-scale digester operated at 35 ℃ indicated the productions of hydrogen, volatile fatty acids (VFAs), ethanol and biogas were inhibited by the added Cu2 + which caused a reduction in the glucose degradation efficiency. The specific yields of hydrogen, ethanol and acetate decreased with increased Cu2+ concentration in the mixed microorganisms. When the Cu2 + concentration in the mixed microorganisms was almost an constant, the specific yields of hydrogen, ethanol and acetate were very similar. A 50% inhibition of hydrogen yield (ECso) occurred at a Cu2+ dosage of 0. 50 mg/L in the present investigation. Cu2+ in the external environment is accumulated in the mixed microorganisms. The Cu2 + speciation, using a sequential extraction technique, showed that the Cu2+ was present mainly bound to organic sites in the mixed microorganisms in the present investigation.
作 者： 任南琪 丁杰 丁兰 刘敏 李永峰 包红旭 作者单位： 任南琪,丁杰,刘敏,李永峰,包红旭(School of Municipal and Environmental Engineering,Harbin Institute of Technology,Harbin 150090,China)丁兰(School of Municipal and Environmental Engineering,Harbin Institute of Technology,Harbin 150090,China;College of Chemistry,Jilin University,Changchun 130023,China)
刊 名： 哈尔滨工业大学学报(英文版) EI 英文刊名： JOURNAL OF HARBIN INSTITUTE OF TECHNOLOGY(NEW SERIES) 年，卷(期)： 2004 11(1) 分类号： X172 关键词： hydrogen fermentation anaerobic digestion Cu2+ volatile fatty acids