Effects of Panax notoginseng saponins on hydrogen peroxide-induced apoptosis in cultured rabbit bone marrow stromal cell
Objective To investigate the effects of Panax notoginseng saponins (PNS) on hydrogen peroxide (H2O2)-induced apoptosis in cultured rabbit bone marrow stromal cells (BMSCs). Methods BMSCs from 3-month-old New Zealand rabbits were isolated and cultured by the density gradient centrifugation combined with adherent method. The cultured BMSCs were divided into three groups: normal control, H2O2 treatment (100?μmol/L), and PNS pretreatment (0.1?g/L). Intracellular reactive oxygen species (ROS) levels as the index of oxidative stress were measured by using 2'7'-dichlorodihydrofluorescein diacetate. Flow cytometry was used to observe the apoptosis of BMSCs by staining with annexinV-FITC/PI. The protein expression of Bax in BMSCs was analyzed by Western blotting. Activity of caspase-3 enzyme was measured by spectrofluorometry. Results Pretreatment with PNS significantly decreased intracellular ROS level induced by H2O2 (P<0.01). PNS markedly attenuated H2O2-induced apoptosis rate from 38.68% to 19.24%(P<0.01). PNS reversed H2O2-induced augmentation of Bax expression. Furthermore, PNS markedly reduced the altered in activity of caspase-3 enzyme induced by H2O2(P<0.01). Conclusion PNS has a protective effect on hydrogen peroxide-induced apoptosis in cultured rabbit BMSCs by scavenging ROS and decreasing Bax expression and caspase-3 activity.
作 者： Hui Qiang Guang-Sheng Wang Chen Zhang Zhi-Bin Shi Li-Hong Fan Kun-Zheng Wang 作者单位： Hui Qiang(Department of Orthopedics, the Second Affiliated Hospital, Medical School of Xi'an Jiaotong University, Xi'an 710004;Department of Orthopedics, Shaanxi Province People's Hospital, Xi'an 710068)Guang-Sheng Wang(Department of Orthopedics, Huashan Hospital, Baoji 721000, China)
Chen Zhang,Zhi-Bin Shi,Li-Hong Fan,Kun-Zheng Wang(Department of Orthopedics, the Second Affiliated Hospital, Medical School of Xi'an Jiaotong University, Xi'an 710004)
刊 名： 西安交通大学学报（英文版） 英文刊名： ACADEMIC JOURNAL OF XI'AN JIAOTONG UNIVERSITY(ENGLISH EDITION) 年，卷(期)： 2010 22(1) 分类号： U4 关键词： Panax notoginseng saponins reactive oxygen species bone marrow stromal cell apoptosis Bax