Obtaining the transgenic poplars with low lignin content through down-regulation of 4CL
The antisense 4CL (4-coumarate: CoA ligase) gene was transformed into triploid Chinese white poplar (Populus tomentosa) mediated by Agrobacterium tumefaciens. PCR and Southern blot analysis indicated that antisense 4CL gene had been integrated into the genome of the transgenic Chinese white poplars. The antisense gene had also been expressed, which was indicated by RT-PCR and Western analysis. Klason lignin content assay showed that repression of 4CL expression could result in remarkable reduction of lignin content in transgenic poplars, with most reduction of 41.73% compared with that of wild type in this paper. But there is no significant difference in holocellulose content between trans- genic and wild poplars. We considered that 4CL might not be the metabolism control point between lignin and carbohy- drate biosynthesis. The stems of transgenic poplars displayed red-brown color with different levels after the bark was peeled, while those of untransformed plants were white. No visible differences in growth and development were observed between transgenic and wild plants. Wiesner reaction analysis of the transgenic plant stems with reduced lignin content exhibited red color, while that of untransformed plant was typically purple-red.
作 者： JIA Caihong ZHAO Huayan Wang Hongzhi XING Zhifeng Du Kejiu SONG Yanru WEI Jianhua 作者单位： JIA Caihong,Wang Hongzhi,XING Zhifeng,Du Kejiu,WEI Jianhua(Beijing Agro-Biotechnology Research Center, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100089, China)ZHAO Huayan,SONG Yanru(Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China)
刊 名： 科学通报(英文版) SCI 英文刊名： CHINESE SCIENCE BULLETIN 年，卷(期)： 2004 49(9) 分类号： Q94 关键词： triploid Chinese white poplar 4CL lignin holocellulose Wiesner reaction