Detecting chimeric 5′/3′UTRs with cross-chromosomal splicing by bioinformatics
The 5′/3′ UTRs of mRNA are crucial in translational regulation, and several serious diseases are believed to be associated with abnormal splicing of these parts of the mRNA sequence. In this work a novel method which uses sequence alignment database searching for detecting chimeric 5′3′ UTRs with cross-chromosomal splicing is reported. Eight highly credible instances of cross-chromosomal splicing have been found using this method, representing additional confirmation of the existence of cross-chromosomal splicing events provided by bioinformatics tools. Since no conserved motif has been found in any of the eight instances, and at the same time current prediction algorithms produce only trivial secondary structures at the "splicing sites", it is not possible to identify any specific signal leading to the splicing.
作 者： ZHANG Zhihua ZHANG Yong SHI Baochen DENG Wei ZHAO Yi CHEN Runsheng 作者单位： ZHANG Zhihua,ZHANG Yong,SHI Baochen,DENG Wei(Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China)ZHAO Yi(Institute of Computing Technology, Chinese Academy of Sciences, Beijing 100080, China)
CHEN Runsheng(Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China;Institute of Computing Technology, Chinese Academy of Sciences, Beijing 100080, China)
刊 名： 科学通报（英文版） SCI 英文刊名： CHINESE SCIENCE BULLETIN 年，卷(期)： 2004 49(10) 分类号： Q3 关键词： 5′/3′ UTRs mRNA trans-splicing chromosomal trans- locations chimeric