Morphological and molecular characterization of two G.Somalense monosomic alien addition lines(MAALs)
Two G. somalense monosomic alien addition lines were identified from the derived backcross progenies of allohexaploid between G. hirsutum and G. somalense through cytological and morphological observation. Furthermore, the alien addition chromosome was identified and distinguished by RAPD analysis. A total of 160 RAPD primers were used for PCR amplification. Primer SBSG11 could produce a specific molecular marker (600 bp) for monosomic alien addition line Ⅰ(MAAL Ⅰ). Primer SBSC03 could produce a specific molecular marker (700 bp) for monosomic alien addition line Ⅱ(MAAL Ⅱ). SBSE07 and SBSE08 could respectively produce common molecular marker for monosomic alien addition lines Ⅰ and Ⅱ. G . somalense alien addition lines could be important for cotton improvement.
作 者： Zhou Zhonghua YU Ping Liu Guanghui HE Jianxing CHEN Jinxiang Zhang Xinxue 作者单位： Zhou Zhonghua(Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China;Institute of Cotton, Hunan Agricultural University, Changsha 410128, China)YU Ping,Liu Guanghui,HE Jianxing,Zhang Xinxue(Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China)
CHEN Jinxiang(Institute of Cotton, Hunan Agricultural University, Changsha 410128, China)
刊 名： 科学通报(英文版) SCI 英文刊名： CHINESE SCIENCE BULLETIN 年，卷(期)： 2004 49(9) 分类号： Q1 关键词： G. hirsutum G. somalense MALLs morphology cytology RAPD