Expression and localization of VCX/Y

Expression and localization of VCX/Y proteins and their possible involvement in regulation of ribosome assembly during s

Variable Charge X/Y (VCX/Y) is a human testis-specific gene family that localized on X and Y chromo-somes. In this study, VCY protein was expressed in E. coli in the form of glutathione-S-transferase (GST)fusion protein. With the purified fusion protein as antigen, the anti-GST-VCY antibody was generated andthe localization of VCY protein in human testis was determined by immunohistochemistry. In the testisseminiferous epithelium, VCY proteins were highly expressed in nuclei of germ cells. Using propidium io-dide staining and green fluorescent protein (GFP) tag technologies, VCY and VCX-8r proteins were mainlylocalized in the nucleoli of COS7 cells. In addition, the colocalization for VCY and VCX-8r in COS7 cellswas also observed. With VCY cDNA as bait, a cDNA fragment of acidic ribosomal protein PO was obtainedusing yeast two-hybrid system. All the information above indicates that VCX/Y protein family might beinvolved in the regulation of ribosome assembly during spermatogenesis.

作 者： SHENG WEI ZOU JIAN CHAO ZHANG Xiao Dong ZHANG Shi Ying MIAO SHU DONG ZONG QI SHENG Lin Fang WANG   作者单位： SHENG WEI ZOU,Xiao Dong ZHANG,Shi Ying MIAO,QI SHENG,Lin Fang WANG(National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Peking Union Medical College, 5 Dong Dan 3-tiao,Beijing 100005, China)

JIAN CHAO ZHANG,SHU DONG ZONG(National Research Institute for Family Planing, WHO Collaboration Center for Research in Human Reproduction,12 Da Hui Si,Beijing 100081, China) 

刊 名： 细胞研究(英文版)  ISTIC SCI 英文刊名： CELL RESEARCH  年，卷(期)： 2003 13(3)  分类号： Q2  关键词： VCX/Y protein   spermatogenesis   nucleoli   colocalization  

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