Isolation and Identification of three Lactobacilli and Research on Antimicrobia Activity in Vitro
Collected from the broiler cecal contents, bacteria were isolated and purified. These isolated and purified Lactobacilli were detected by acidity and bile salt tolerant test, then three better tolerant strains were screened out. These three strains were conducted to observe the morphous through microscope, perform Gram stain, and to identify their physiological and biochemical characteristics. The results showed that L1 was L.casei, L2 was L.gasseri and L3 was L.graminis. With Oxford Cup mcthod, these three Lactobacilli were detected for their bacteriostasis. Results showed that L1, L2 and L3 strains had strong antibacterial activities to E. coli and Salmonella.
作 者： CHEN Xin XU Li 作者单位： College of Animal Science and Technology,Northeast Agricultural University,Harbin 150030,China 刊 名： 东北农业大学学报（英文版） 英文刊名： JOURNAL OF NORTHEAST AGRICULTURAL UNIVERSITY(ENGLISH EDITION) 年，卷(期)： 2009 16(2) 分类号： QS Q517 关键词： Lactobacillus isolation and identification in vitro antibiosis