OsDREB1 Gene from Rice Enhances Cold Tolerance in Tobacco
The OsDREB1 gene from rice encodes a transcription factor belonging to the DREBP transcription factor subfamily. Many DREBP transcription factors regulate gene expression in response to drought, high-salt, and cold stresses by binding specifically to the dehydration-responsive element (DRE). DRE-binding proteins, such as CBF1, DREB1A, and DREB2A, have been cloned from Arabidopsis thaliana and have been proved to play an important role in stress response of Arabidopsis and several other plants. In this study, the OsDREB1 gene was transferred to tobacco plants by the Agrobacterium-mediated transfer method, and 16 transgenic plants were identified. PCR analysis demonstrates that the foreign genes have been integrated into the tobacco genome. Results of freezing stress experiments indicate that the transgenic plants have enhanced cold tolerance.
作 者： LI Ping CHEN Feng QUAN Chao ZHANG Guiyou 作者单位： Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, China 刊 名： 清华大学学报（英文版） EI 英文刊名： TSINGHUA SCIENCE AND TECHNOLOGY 年，卷(期)： 2005 10(4) 分类号： Q3 关键词： transformation OsDREB1 transcription factor cold stress