Cloning of a down-regulated gene encoding small GTP binding protein in hybrid wheat
Previous studies showed that differential gene expression between wheat hybrids and their parents was responsible for the heterosis. To provide an insight into the molecular basis of wheat heterosis, one cDNA, designated TaRab, was identified from the cDNA library of wheat seedling leaves. The sequence comparison in GenBank revealed that TaRab is homologous to a group of genes encoding Rab-GTP binding protein. Semi-quantitative RT-PCR analysis indicated that TaRab was expressed in all plant tissues examined,but at slightly higher level in leaves. Further analysis exhibited that TaRab displayed lower expression in hybrid than in its patents in both roots and leaves, which was in agreement with the original results of suppression subtractive hybridization. TaRab was located on chromosome 7B and C-7DS5-0.36 by in silico mapping. The relationship between differential expression of TaRab and the molecular basis of wheat heterosis was also discussed.
作 者： YAO Yingyin NI Zhongfu CHEN Rongmin Wu Limin SUN Qixin 作者单位： Department of Plant Genetic & Breeding, State Key Laboratory for Agro-biotechnology, China Agricultural University, Beijing 100094,China 刊 名： 自然科学进展（英文版） SCI 英文刊名： PROGRESS IN NATURAL SCIENCE 年，卷(期)： 2005 15(7) 分类号： Q5 关键词： wheat Rab protein gene expression heterosis