Studies on the Interaction of Beryllon Ш with Proteins by Voltammetric Technique and its Application
A new quantitative determination method of proteins using beryllon Ш by voltammetric technique was developed in this paper. In pH 3.5 Britton-Robinson (B-R) buffer solution, beryllon Ш can bind with human serum albumin (HSA) to form an electro-inactive supermolecular complex. Beryllon Ш has a well-defined voltammetric reduction peak at -0.38 V (vs. SCE) and the addition of protein in this solution can cause the decrease of the reductive peak current. Based on the decrease of the reduction peak current, a new electrochemical method for the determination of HSA was established with linear range of 1.0~40.0 mg/L and the detection limit of 1.0 mg/L. This method is further applied to the determination of real sample of healthy human serum.
作 者： Wei SUN Kui JIAO Xue Liang WANG Lu De LU 作者单位： Wei SUN(College of Chemical Engineering, Nanjing University of Science and Technology, Nanjing 210094;College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042)Kui JIAO,Xue Liang WANG(College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042)
Lu De LU(College of Chemical Engineering, Nanjing University of Science and Technology, Nanjing 210094)
刊 名： 中国化学快报(英文版) ISTIC SCI 英文刊名： CHINESE CHEMICAL LETTERS 年，卷(期)： 2003 14(12) 分类号： O6 关键词： Beryllon Ш serum albumin voltammetry binding reaction