Electrochemical Studies of the Recognition Interaction of Rhodamine B with DNA
The recognition interaction of rhodamine B (RB) with DNA was studied in pH 7.5Britton-Robinson (B-R) buffer solution by electrochemical techniques. An irreversible oxidation peak at glassy carbon electrode was obtained at +0.92V (vs. SCE). After the addition of DNA into the RB solution, the peak current of RB decreased apparently without the shift of peak potential. The electrochemical parameters such as the charge transfer coefficient α and the electrode reaction standard rate constant ks of RB in the absence and presence of DNA were determined, which did not change, indicating that a non-electroactive complex was formed, so the concentration of RB in the solution decreased and the peak current decreased correspondingly.
作 者： Kui JIAO Qing Jun LI Wei SUN Zhen Yong WANG 作者单位： College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology,Qingdao 266042 刊 名： 中国化学快报（英文版） ISTIC SCI 英文刊名： CHINESE CHEMICAL LETTERS 年，卷(期)： 2005 16(3) 分类号： 关键词： Rhodamine B DNA recognition interaction electrochemistry