Determination of Metformin in Human Plasma by Liquid Chromatography-tandem Mass Spectrometric Assay
Introduction Metformin (1,1-dimethylbiguanide) (Fig. 1) is an oral anti-hyperglycemic agent used in the treatment of non-insulin-dependent diabetes mellitus (type Ⅱ). Owing to its weight-decreasing and serum lipid-normalizing effects, it is especially recommended for obese patients[1,2] Various analytical methods have been described for the measurement of metformin in biological fluids, including gas chromatography(GC)[3-5], capillary electrophoresis (CE)[6] and HPLC[7～16]with UV detection[7-12,17] HPLC-Mass spectrometry (LC/APCIMS/MS ) offers an attractive alternative to HPLC[18].
作 者： WANG Jian WANG Ying-wu GU Jing-kai WU Yi WANG Yan 作者单位： Research Center for Drug Metabolism, Jilin University, Changchun 130023, P. R. China 刊 名： 高等学校化学研究（英文版） ISTIC SCI 英文刊名： CHEMICAL RESEARCH IN CHINESE UNIVERSITIES 年，卷(期)： 2005 21(2) 分类号： O6 关键词： Metformin LC-MS-MS Human plasma