Optimization of Culturing Condition and Medium Composition for the Production of Alginate Lyase by a Marine Vibrio sp. Y
Carbohydrases secreted by marine Vibrio sp. YKW-34 with strong Laminaria cell wall degrading ability were screened,and among them alginate lyase was found to be dominant. The effects of medium composition and culturing condition on the production of alginate lyase by marine Vibrio sp. YKW-34 in flask were investigated in this study. In the culture medium of marine broth, no alginate lyase was produced. The activity of the alginate lyase, after being induced, reached 5 UmL-1. The best inoculum volume and inoculum age were 10% and 12 h, respectively. The optimal temperature for alginate lyase production was 25℃. The fermentation medium was composed of 0.5% of Laminaria powder and 0.2% of KNO3 with an initial acidity of pH 8.0. Alginate could induce alginate lyase production but not as efficiently as Laminaria powder did. The addition of fucoidan, cellulose and glucose had negative effect on the alginate lyase production. Other kinds of nitrogen sources, such as yeast extract, beef extract and peptone, had positive effect on the growth of the microorganism and negative effect on alginate lyase production. In addition, the time course of alginate lyase production under the optimized condition was described. The optimal harvest time was 48 h.
作 者： FU Xiaoting LIN Hong KIM Sang Moo 作者单位： FU Xiaoting(College of Food Science and Engineering, Ocean University of China, Qingdao 266003, P. R. China;Faculty of Marine Bioscience and Technology, Kangnung National University, Gangneung, 210-702, Korea)LIN Hong(College of Food Science and Engineering, Ocean University of China, Qingdao 266003, P. R. China)
KIM Sang Moo(Faculty of Marine Bioscience and Technology, Kangnung National University, Gangneung, 210-702, Korea)
刊 名： 中国海洋大学学报（英文版） ISTIC 英文刊名： JOURNAL OF OCEAN UNIVERSITY OF CHINA 年，卷(期)： 2008 7(1) 分类号： P7 关键词： alginate lyase Vibrio sp. YKW-34 medium optimization