Analysis of SSRs Derived from an EST Library of Half-Smooth Tongue Sole Cynoglossus semilaevis
A complementary DNA (cDNA) library was constructed from half-smooth tongue sole spleen. A long-read expressed sequence tag (EST) database was generated, containing 3100 cDNA clones, of which 220 clones were fully sequenced. A total of 1060 non-redundant simple sequence repeats (SSRs) were obtained from the cDNA library. An average of 5 kb sequence generates 1 SSR in the half-smooth tongue sole spleen cDNA library. The proportion of the SSR unit size was different in the cDNA library. The monomeric repeats (51.4%) are the most abundant class of SSR in the dataset. The dimeric, trimeric, tetrameric and hexameric re- peats are represented in decreasing proportions of 27.2%, 16.0%, 2.8% and 1.9%, respectively. The frequency of pentameric repeats was observed the least (only 0.7%). Most of the monomeric and dimeric repeats are distributed in 3' and 5' un-translation region. If translation regions are considered merely, trimeric repeats are the highest, accounting for 57% of the total microsatellites.
作 者： LIU Yunguo BAO Baolong CHEN Songlin SUN Xiuqin 作者单位： LIU Yunguo(College of Fisheries, Ocean University of China, Qingdao 266003, P. R. China;Key Laboratory of Marine and Estuarine Fisheries, Ministry of Agriculture, East China Sea Fisheries Research Institute, Shanghai 200090, P. R. China)BAO Baolong(College of Aqua-Lite Science and Technology, Shanghai Ocean University, Shanghai 200090, P. R. China)
CHEN Songlin(Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, P. R. China)
SUN Xiuqin(The First Institute q/Oceanography, State Oceanic Administration, Qingdao 266061, P. R. China)
刊 名： 中国海洋大学学报（英文版） ISTIC 英文刊名： JOURNAL OF OCEAN UNIVERSITY OF CHINA 年，卷(期)： 2008 7(3) 分类号： P7 关键词： half-smooth tongue sole Cynoglossus semilaevis EST microsatellite