Death of Prorocentrum donghaiense May Be a Programmed Autolysis
Prorocentrum donghaiense, one of the dinoflagellate, has continuously caused large scale red tides along the Chinese coast in recent years. These red tides have brought tremendous loss to the local economy and serious impacts to the local environ- ment. Unfortunately, little was known about the mechanism of the fast appearance and extinction of the red tide caused by this alga. In this study, the full-length cDNA of a caspase encoding gene ofP donghaiense was cloned and characterized, and the transcription of this gene during the senescence of the alga was semi-quantitatively determined. The cDNA was 520bp in length. It contained a 258bp open reading frame (ORF) which encoded a peptide of 85 amino acids. The amount of transcripts of the caspase encoding gene increased with the senescence of P. donghaiense and started to decrease gradually when the autolysis of P. donghaiense cells took place. We proposed that the death of P. donghaiense may be a caspase mediated programmed autolysis.
作 者： YANG Guanpin LIU Yongjian LI Bingjun 作者单位： YANG Guanpin,LIU Yongjian(College of Marine Life Sciences, Ocean University of China, Qingdao 266003, P. R. China)LI Bingjun(College of Marine Life Sciences, Ocean University of China, Qingdao 266003, P. R. China;College of Oceanography, Yantai University, Yantai 264005, P. R. China)
刊 名： 中国海洋大学学报（英文版） ISTIC 英文刊名： JOURNAL OF OCEAN UNIVERSITY OF CHINA 年，卷(期)： 2008 7(3) 分类号： P7 关键词： caspase programmed autolysis Prorocentrum donghaiense RT-PCR