Study of Immunoassay Methods for Recombinant Human Erythropoietin(rhEPO)Using Competitive ELISA
Two different immunoassay methods, competitive indirect enzyme-linked immuno- sorbent assay (CI-ELISA) and amplificative competitive indirect ELISA (ACI-ELISA) using biotin-avidin complex system were studied to detect rhEPO.The linear ranges were 50-20000 ng/mL and 10-50000 ng/mL for CI-ELISA and ACI-ELISA, respectively.The low detection limits of CI-ELISA and ACI-ELISA were 62.8 ng/mL and 8.5 ng/mL, respectively.
作 者： Jin YAN Jie Bo MI Wen Bao CHANG 作者单位： The Key Laboratory of Bioorganic Chemistry and Molecular Engineering, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871 刊 名： 中国化学快报(英文版) ISTIC SCI 英文刊名： CHINESE CHEMICAL LETTERS 年，卷(期)： 2004 15(8) 分类号： 关键词： Recombinant human erythropoietin (rhEPO) polyclonal antibody (pAb) competitive indirect ELISA (CI-ELISA) amplificative competitive indirect ELISA (ACI-ELISA)