The Prokaryotic Expression and Bioactivity of the Recombinant Red Fire Ant Venom Allergen Soli4
The sting of red imported fire ant (RIFA) could cause serious allergic response in fraction of people. These allergic reactions are mainly caused by its venom, especially venom allergen Sol I 1-4. To produce large amount of RIFA venom allergen Sol I 4 for diagnosis of RIFA allergy and allergen-specific immunotherapy, the gene encoding this protein was amplified and cloned into the prokaryotic expression vector pET43, la. The recombinant plasmid was used to transform competent cells and the recombinant proteins were expressed in E. Coli. SDS-PAGE and Western blotting analysis indicated that high-level expression of Sol I 4 protein was successfully achieved. Allergenic activity analysis of the recombinant allergen Sol I 4 was then performed on rabbit. The result showed that the recombinant protein obtained had significant allergenic activity. It indicated that the recombinant allergen Sol I 4 of RIFA venom was successfully expressed in E. Coli, which provided foundation for further developing therapeutic and diagnosis reagents of RIFA allergy.
作 者： HAN Xue-qing LIN Xiang-mei CHEN Hong-jun ZHANG Yong-guo YE Gui-sheng WU Shao-qiang LI Jian CHEN Nai-zhong CHEN Yan ZHU Shui-fang 作者单位： Chinese Academy of Inspection and Quarantine, Beijing 100029, P.R.China 刊 名： 中国农业科学（英文版） 英文刊名： AGRICULTURAL SCIENCES IN CHINA 年，卷(期)： 2009 8(2) 分类号： S1 关键词： red imported fire ant recombinant allergen Sol i 4 expression activity