A Method for Identification of Selenoprotein Genes in Archaeal Genomes
The genetic codon UGA has a dual function: serving as a terminator and encoding selenocysteine. However, most popular gene annotation programs only take it as a stop signal, resulting in misannotation or completely missing selenoprotein genes. We developed a computational method named Asec-Prediction that is specific for the prediction of archaeal selenoprotein genes. To evaluate its effectiveness, we first applied it to 14 archaeal genomes with previously known selenoprotein genes, and Asec-Prediction identified all reported selenoprotein genes without redundant results. When we applied it to 12 archaeal genomes that had not been researched for selenoprotein genes, Asec-Prediction detected a novel selenoprotein gene in Methanosarcina acetivorans. Further evidence was also collected to support that the predicted gene should be a real selenoprotein gene. The result shows that Asec-Prediction is effective for the prediction of archaeal selenoprotein genes.
作 者: 作者单位: 刊 名: 基因组蛋白质组与生物信息学报(英文版) 英文刊名: GENOMICS、PROTEOMICS & BIOINFORMATICS 年,卷(期): 2009 ""(1) 分类号: Q5 关键词: archaea selenocysteine selenoprotein SECIS SelB gene prediction