Characteristics of the binding features of Nelin with F-actin and screening Nelin interactive proteins
The interaction of nelin, a cardiac-specific expressed protein of human novel gene nelin, with F-actin was studied by both F-actin cosedimentation in vitro and colocal-ization assays.The results showed that nelin is a new F-actin binding protein and is colocolized with F-actin in cytoplasm of cells.Three new nelin binding proteins, filamin C subtype, titin N2B subtype and inter-alpha trypsin inhibitor heavy chain precursor (ITIH), were identified from human heart cDNA library using yeast two-hybrid screening.The binding activity of filamin C with nelin was confirmed by coim-munoprecipitation.Filamin C binds to nelin through its C-terminal region.It is indicated that nelin is a cytoskeleton associated protein and acts as a membrane-cytoskeleton as-sociated protein involved in the formation of focal adhesions.
作 者： WANG Zhen Meng Xianmin CAO Huiqing LIU Dongqing FENG Yan YOU Xin WANG Yin GUO LianJun QU Shen 作者单位： WANG Zhen(Molecular Medical Center, Cardiovascular Institute and Fu Wai Hospital, CAMS and PUMC, Beijing 100037, China;Department of Molecular Biology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China)Meng Xianmin,CAO Huiqing,LIU Dongqing(Molecular Medical Center, Cardiovascular Institute and Fu Wai Hospital, CAMS and PUMC, Beijing 100037, China)
FENG Yan,WANG Yin,GUO LianJun(School of Basic Medical Sciences, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China)
YOU Xin(Department of Inspection, Affiliated Hospital of Medical College, Yanbian University, Yanji 133000, China)
QU Shen(Department of Molecular Biology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China)
刊 名： 科学通报（英文版） SCI 英文刊名： CHINESE SCIENCE BULLETIN 年，卷(期)： 2004 49(23) 分类号： Q5 关键词： Nelin F-actin yeast two-hybrid coimmunoprecipitation cytoskeleton