RAPD Anlysis of Different Leaf Colou

RAPD Anlysis of Different Leaf Colour in Euphorbia pulcherrima

Random amplified polymorphic DNA (RAPD) was used to study the diversity of seven E. pulcherrima with different color leaves. Aight 10 bp primers selected from 30 arbitrary primers were applied for amplifying the Euphorbia pulcherrima DNA. Total 62 bands were produced, among which 22 bands (35.48%) were polymorphic. The average numbers of polymorphic DNA bands amplified by each primer were 7.8. Cluster analysis results showed that the genetic background of seven E. pulcherrima was relatively narrow and they had the higher similarity coefficient, which indicated that these seven strains had a near relationship, and that RAPD marking technology may be the effective means for E. pulcherrima classification.

作 者： BAI Heine NIU De WANG Lijuan   作者单位： College of Life Sciences,Northeast Agricultural University,Harbin 150030,China  刊 名： 东北农业大学学报（英文版）  英文刊名： JOURNAL OF NORTHEAST AGRICULTURAL UNIVERSITY(ENGLISH EDITION)  年，卷(期)： 2009 16(2)  分类号： Q31  关键词： E. pulcherrima   RAPD   cluster analysis  

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