The relationship between LDL oxidation and macrophage myeloperoxidase activity
Objective To explore low density lipoprotein (LDL) oxidation by macrophage myeloperoxidase (MPO) at molecular level.Methods Using a mouse macrophage model, we examined the relationship between LDL oxidation and macrophage MPO by measuring macrophage MPO activity, LDL oxidation products, MPO gene expression and cellular orientation of LDL oxidation. Results MPO gene expression increased to its maximum gradually when the concentration of LDL was increased, and then maintained at that level. NaN3 inhibied the elevation of MPO activity and LDL oxidation, which was LDL concentration-dependent. After the composition of macrophage membrane was roughly analyzed, it was determined that the contents of MPO and LDL in 5% sucrose were 7.667 and 21 times higher than those in 10% sucrose, respectively. Conclusion LDL is attached to the "microdomain" of the macrophage membrane in which LDL is oxidized by MPO.
作 者： 武军驻 刘艳红 李小明 陈丽达 夏腊菊 洪嘉玲 作者单位： Department of Biochemistry, College of Medicine, Wuhan University, Wuhan 430071, China 刊 名： 中华医学杂志(英文版) ISTIC SCI 英文刊名： CHINESE MEDICAL JOURNAL 年，卷(期)： 2003 116(5) 分类号： Q5 关键词： low density lipoprotein oxidation myeloperoxidase