Gene expression profiles of human bone marrow derived mesenchymal stem cells and tendon cells
Objective To study the gene expression profiles of human bone marrow derived mesenchymal stem cells and tendon cells.Methods Total RNA extracted from human bone marrow derived mesenchymal stem cells and tendon cells underwent reverse transcription, and the products were labeled with α-32P dCTP. The cDNA probes of total RNA were hybridized to cDNA microarray with 1176 genes, and then the signals were analyzed by AtlasImage analysis software Version 1.01a.Results Fifteen genes associated with cell proliferation and signal transduction were up-regulated, and one gene that takes part in cell-to-cell adhesion was down-regulated in tendon cells.Conclusion The 15 up-regulated and one down-regulated genes may be beneficial to the orientational differentiation of mesenchymal stem cells into tendon cells.
作 者： 胡庆柳 朴英杰 邹飞 作者单位： 胡庆柳,邹飞(Department of Thermal Environmental Medicine, First Military Medical University, Guangzhou 510515, China)朴英杰(Department of Anatomy, First Military Medical University, Guangzhou 510515, China)
刊 名： 中华医学杂志(英文版) ISTIC SCI 英文刊名： CHINESE MEDICAL JOURNAL 年，卷(期)： 2003 116(8) 分类号： Q7 关键词： cDNA microarray gene expression stem cells tendon cells