Survival of the biocontrol agents Brevibacillus brevis ZJY-1 and Bacillus subtilis ZJY-116 on the spikes of barley in th
Fusarium head blight (FHB) caused by Fusarium graminearum is a devastating disease that results in extensive yield losses to wheat and barley. A green fluorescent protein (GFP) expressing plasmid pRP22-GFP was constructed for monitoring the colonization of two biocontrol agents, Brevibacillus brevis ZJY-1 and Bacillus subtilis ZJY-116, on the spikes of barley and their effect on suppression of FHB. Survival and colonization of the Brevibacillus brevis ZJY- 1 and Bacillus subtilis ZJY- 116 strains on spikes of barley were observed by tracking the bacterial transformants with GFP expression. Our field study revealed that plasmid pRP22-GFP was stably maintained in the bacterial strains without selective pressure. The retrieved GFP-tagged strains showed that the bacterial population fluctuation accorded with that of the rain events. Furthermore, both biocontrol strains gave significant protection against FHB on spikes of barley in fields. The greater suppression of barley FHB disease was resulted from the treatment of barley spikes with biocontrol agents before inoculation with F. graminearum.
作 者： ZHANG Xin ZHANG Bing-xin ZHANG Zhen SHEN Wei-feng YANG Ching-hong YU Jing-quan ZHAO Yu-hua 作者单位： ZHANG Xin,ZHANG Bing-xin(Department of Plant Protection, School of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310029, China)ZHANG Zhen,SHEN Wei-feng(Academy of Agriculture Science, Hangzhou 310021, China)
YANG Ching-hong(Department of Biological Sciences, University of Wisconsin-Milwaukee, WI 53211, USA)
YU Jing-quan(Department of Horticulture, School of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310029, China)
ZHAO Yu-hua(School of Life Science, Zhejiang University, Hangzhou 310029, China)
刊 名： 浙江大学学报B（英文版） SCI 英文刊名： JOURNAL OF ZHEJIANG UNIVERSITY SCIENCE B 年，卷(期)： 2005 6(8) 分类号： Q78 关键词： GFP Survival Brevibacillus brevis and Bacillus subtilis Spikes Barley Biocontrol