动态超高压微射流对卵清蛋白微观结构的影响

动态超高压微射流对卵清蛋白微观结构的影响

摘要：采用圆二色谱(CD)、X射线衍射(XRD)、ANS荧光探针和紫外(UV)光谱研究了动态超高压微射流对卵清蛋白微观结构的影响.结果表明:卵清蛋白的微观结构的变化与处理压力有关,CD显示不同压力处理的卵清蛋白二级结构中的α-螺旋,β-折叠,β-转角和无规卷曲之间发生相互转化,二级结构的有序性提高;X射线衍射图谱直观显示不同压力处理的卵清蛋白品体结构增加,160 MPa处理下结晶区最大,说明蛋白结构的有序性提高,与CD分析结果相似;ANS荧光探针光谱显示卵清蛋白的表面疏水性随着处理压力的`增大而提高,120 MPa处理下达到最大;紫外光谱显示随着处理压力的增大,卵清蛋白最大紫外吸光值下降,卵清蛋白分子表面的具有紫外吸收的芳香氨基酸残基被包埋于分子内部,卵清蛋白的三维结构发生改变.Abstract：The effect of dynamic high pressure microfluidization on the microstructure of ovalbumin was studied by CD spectra,XRD spectra,ANS fluorescence probe emission spectra and UV absorption spectra.The results indicated that the changes in the microstructure were dependent on the pressure.CD spectra were used to examine the changes in the secondary structure of the ovalbumin treated by different pressures.When the pressure increased,the mutual transformation between α-helix,β-sheet,β-tum and the random coil was observed.The orderliness of the secondary structure was increased with increasing the pressure.XRD spectra analysis showed that the crystal structure content of the ovalbumin treated increased with increasing the pressure and the largest data was observed at 160 MPa,indicating that the orderliness of the secondary structure was increased.The results were similar to CD spectra analysis.The ANS fluorescence probe emission spectra analysis demonstrated that the dynamic high pressure microfluidization induced an increase in surface hydrophobicity following high pressure treatment,while the largest data was observed at 120 MPa.In addition,UV absorption spectra analysis indicated that dynamic high pressure microfluidization treatment also resulted in a decrease in the UV-absorption maximum wavelength with increasing the pressure,indicating that the aromatic amino acid was buried in the molecular interior and the three dimensional structure of ovalbumin was changed. 作者： 涂宗财王辉刘光宪陈钢豆玉新张雪春 Author： TU Zong-cai  WANG Hui  LIU Guang-xian  CHEN Guang  DOU Yu-xin  ZHANG Xue-chun 作者单位： 南昌大学食品科学与技术国家重点实验室,江西,南昌,330047 期 刊： 光谱学与光谱分析   ISTICEISCIPKU Journal： SPECTROSCOPY AND SPECTRAL ANALYSIS 年，卷(期)： 2010, 30(2) 分类号： Q0631.1 关键词： 动态超高压微射流    卵清蛋白    圆二色谱    X射线衍射    ANS荧光探针    紫外光谱    Keywords： Dynamic high pressure microfluidization    Ovalbumin    Circular dichroism    XRD spectra    ANS fluorescence probe    Ultraviolet spectra    机标分类号： R91 R9 机标关键词： 动态    超高压微射流    卵清蛋白    微观结构    Microstructure    Treatment    High Pressure    absorption spectra    secondary structure    high pressure    fluorescence probe    emission spectra    analysis    压力处理    microstructure    UV absorption    ANS荧光探针    dynamic    aromatic amino acid    treatment 基金项目： 国家自然科学基金，教育部长江学者奖励计划

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