Production of Polyclonal Antibody of Morphine and Determination of Morphine in Urine by Capillary Electrophoresis Immuno
N-Conjugated antigen was synthesized and polyclonal antibody with high specificity was obtained from immunizing animals.With this polyclonal antibody, a rapid and efficient CEIA-LIF method was developed to determine the free morphine in urine of abusers.The detection limit was calculated to be 40 ng/mL.Simulated urine samples were analyzed with good recoveries, which showed the feasibility of its application in specific morphine determination in urine of morphine abusers.
作 者： Jian Qiu MI Xiao Hua QI Xin Xiang ZHANG Wen Bao CHANG 作者单位： The Key Lab of Bioorganic Chemistry and Molecular Engineering, College of Chemistry, Peking University, Beijing 100871 刊 名： 中国化学快报(英文版) ISTIC SCI 英文刊名： CHINESE CHEMICAL LETTERS 年，卷(期)： 2004 15(8) 分类号： 关键词： Polyclonal antibody morphine capillary electrophoresis immunoassay (CEIA) laser-induced fluorescence (LIF) specific