Third Generation Horseradish Peroxidase Biosensor Based on Self-assembling Carbon Nanotubes to Gold Electrode Surface
A third-generation horseradish peroxidase (HRP) biosensor has been developed by adsorbing HRP on multi-wall carbon nanotube (MWNTs) monolayer modified gold electrode surface. The assembly process was investigated by electrochemical and spectroscopic techniques.Results showed that the immobilized HRP exhibited direct electrochemical behavior toward the reduction of H2O2. The resulting biosensor shows a fast amperometric response (＜2 s) to H2O2.The linear response range was from 5.0×10-7～1.0×10-5 mol/L with a detection limit of1.0×10-7mol/L. Moreover, the biosensor has a good reproducibility, and long-term stability.
作 者： Jing Juan XU Gang Wang Qing ZHANG Xing Hua XIA Hong Yuan CHEN 作者单位： The Laboratory of Life Analytical Chemistry, Department of Chemistry, Nanjing University, Nanjing 210093 刊 名： 中国化学快报（英文版） ISTIC SCI 英文刊名： CHINESE CHEMICAL LETTERS 年，卷(期)： 2005 16(4) 分类号： 关键词： Horseradish peroxidase MWNT self-assembly third-generation biosensor