Biosynthesis of Succulent Bamboo Shoots of Bambusa balcooa into Phytosterols and Its Biotransformation into ADD
Fermentation of the succulent bamboo shoots of Bambusa balcooa Roxb. resulted in an enrichment of phytosterols from 0.12% to 0.62% dry weight as compared to that of the fresh unfermented samples. The bacterial strains responsible for higher accumulation of phytosterols during fermentation of the bamboo shoots have been isolated and further extraction and purification of the crude phytosterols (isolated from the fermented samples) were done by TLC, UV, NMR, IR and Mass spectral analysis. The isolated phytosterols (β-sitos-terols) were then subjected to microbial transformation which yielded a considerable amount of androsta-1, 4-diene-3,17-dione (ADD) in the incubation mixture in presence of metabolic inhibitors (α, α＇-dipyridyl and sodium arsenate).
作 者： Kananbala SARANGTHEM Thongam Nabakumar SINGH 作者单位： Plant Physiology Laboratory, Department of Life Sciences, Manipur University, Imphal 795003, India 刊 名： 植物学报 ISTIC SCI 英文刊名： ACTA BOTANICA SINICA 年，卷(期)： 2003 45(1) 分类号： Q9 关键词： Bambusa balcooa biosynthesis phytosterols androsta-1,4-diene-3,17-dione (ADD)