Biological properties of differently-aged human keratinocytes:population doubling time growth curve and cell cycle analy
Objective To explore the biological properties of keratinocytes from differently-aged healthy human beings. Methods Keratinocytes from fetus, teenager and middle-aged groups were separated and cultured. The population doubling time (PDT) and cell growth curve in different cells were compared, and the cell cycles were analyzed by flow cytometry. Results ① In primary culture of keratinocytes, the adherence time in middle-aged group was longer than that in fetus and teenager groups. However, all cell morphology showed no obvioas differences. In subculture of kecatinocytes, with donator's age increasing, time of cell adherence prolonged, passage number decreused and differences in cell morphology were obrioas. ② The average PDT of keratinocytes was shorter in fetus group than in teenager and middle-aged groups. Bat difference in cell growth curve between different passages was not observed. ③ Keratinocytes showed G2/M period in fetus group but G0/G1 period in teenager and middle-aged groups mainly. Conclusion As age increases, the biological properties of keratinocytes change obviously.
作 者： Hui-qun Ma Jie Feng Lech Chyczewski Jacek Niklinski 作者单位： Hui-qun Ma(Department of Dermatology,the Second Affiliated Hospital,Medical School of Xi'an Jiaotong University,Xi'an 710061,China;Department of Clinical Molecular Biology,Bialystok Medical University,Bialystok,Poland)Jie Feng(Department of Dermatology,the Second Affiliated Hospital,Medical School of Xi'an Jiaotong University,Xi'an 710061,China)
Lech Chyczewski(Department of Clinical Molecular Biology,Bialystok Medical University,Bialystok,Poland)
Jacek Niklinski(Department of Thoracic Surgery,Bialystok Medical University,Bialystok,Poland)
刊 名： 西安交通大学学报（英文版） 英文刊名： ACADEMIC JOURNAL OF XI'AN JIAOTONG UNIVERSITY(ENGLISH EDITION) 年，卷(期)： 2009 21(2) 分类号： Q253 关键词： biological property keratinocyte human grow curve cell cycle