Direct MALDI-MS Analysis of Proteins Isolated by Liquidphase Isoelectric Focusing Electrophoresis
A liquid-phase isoelectric focusing electrophoresis system(Rotofor) was used as the prefractionation tool for the sample preparation in the MALDI-MS analysis of a protein mixture. Each fraction collected was then directly subjected to MALDI-TOF-MS analysis. By this approach, we are able to resolve two types of hemoglobins, A and C, which cannot be successfully separated by means of the traditional SDS-PAGE method.
作 者： LIU Ning L(U¨) Lei ZHANG Xuan WANG Zhi SUN Ya-dong LIU Zhi-qiang LIU Shu-ying 作者单位： LIU Ning(Laboratory of New Drug Research and Mass Spectrometry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, P. R. China;Institute of Life Science and Technology, Jilin University, Changchun 130021, P.R. China)L(U¨) Lei,ZHANG Xuan,LIU Zhi-qiang,LIU Shu-ying(Laboratory of New Drug Research and Mass Spectrometry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, P. R. China)
WANG Zhi,SUN Ya-dong(Key Laboratory of Molecular Enzymology and Engineering of Educational Ministry, Jilin University, Changchun 130021, P. R. China)
刊 名： 高等学校化学研究（英文版） ISTIC SCI 英文刊名： CHEMICAL RESEARCH IN CHINESE UNIVERSITIES 年，卷(期)： 2005 21(4) 分类号： O6 关键词： Liquid-phase isoelectric focusing electrophoresis MALDI-MS analysis Hemoglobin A and C Cytochrome c