Lignin reduction in transgenic poplars by expressing antisense CCoAOMT gene
The antisense Caffeoyl CoA O-methyltransferase (CCoAOMT) cDNA was transformed into Chinese white poplar (Populus tomentosa) mediated by Agrobacterium tumefaciens. Many factors affecting the transformation efficiency were studied and a stable transformation system was established. PCR-Southern blot analysis indicated that antisense CCoAOMT cDNA had been integrated into the genome of the transgenic poplars. RT-PCR and Western blot analyses demonstrated that the endogenous CCoAOMT gene was suppressed at both transcriptional and translational levels. Klason lignin content assay exhibited the lignin reduction to different degrees in transgenic poplars. The stems of partial transgenic poplars with the remarkable lignin reduction turned red, and the color distribution was stripped or spotted. Taken together, these results suggested that CCoAOMT gene would be a potential useful gene in altering lignin biosynthesis by biotechnology for improving wood properties.
作 者： LU Jing ZHAO Huayan WEI Jianhua HE Yikun SHI Chao Wang Hongzhi SONG Yanru 作者单位： LU Jing(Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China;Department of Biology, Capital Normal University, Beijing 100037, China)ZHAO Huayan,SHI Chao,SONG Yanru(Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China)
WEI Jianhua,Wang Hongzhi(Beijing Center of Agro-Biotechnology, Beijing Institute of Agriculture and Forestry, Beijing 100089, China)
HE Yikun(Department of Biology, Capital Normal University, Beijing 100037, China)
刊 名： 自然科学进展（英文版） SCI 英文刊名： PROGRESS IN NATURAL SCIENCE 年，卷(期)： 2004 14(12) 分类号： Q3 关键词： Populus tomentosa CCoAOMT Agrobacterium tumefaciens transgene lignin