Expression of a begomoviral DNAβ gene in transgenic Nicotiana plants induced abnormal cell division
An increasing number of monopartite begomoviruses are being identified that a satellite molecule (DNAβ) is required to induce typical symptoms in host plants. DNAβ encodes a single gene (termed βC1) encoded in the complementary-sense. We have produced transgenic Nicotiana benthamiana and N. tabacum plants expressing theβC1 gene of a DNAβ associated with Tomato yellow leaf curl China virus (TYLCCNV), under the control of the Cauliflower mosaic virus 35S promoter. Transgenic plants expressing βC1 showed severe developmental abnormalities in both species. Microscopic analysis of sections of both transgenic and non-transgenic N. tabacum leaves showed abnormal outgrowths of transgenic N. tabacum to be due to disorganized cell division (hyperplasia) of spongy and palisade parenchyma. Immuno-gold labeling of sections with a polyclonal antibody against the βC1 protein showed that the βC1 protein accumulated in the nuclei of cells. The possible biological function of the βC1 protein was discussed.
作 者： CUI Xiao-feng LI Yun-qin HU Dong-wei ZHOU Xue-ping 作者单位： Institute of Biotechnology, Zhejiang University, Hangzhou 310029, China 刊 名： 浙江大学学报B(英文版) SCI 英文刊名： JOURNAL OF ZHEJIANG UNIVERSITY(SCIENCE) 年，卷(期)： 2005 6B(2) 分类号： Q78 关键词： Tomato yellow leaf curl China virus (TYLCCNV) DNAβ βC1 gene Transgenic plant Cell division