Involvement of NO in fungal elicitor-induced activation of PAL and stimulation of taxol synthesis in Taxus chinensis sus
Elicitor prepared from the cell walls of Penicillium citrinum induces multiple responses of Taxus chinensis cells, including nitric oxide (NO) generation, sequentially followed by the activation of PAL and synthesis of taxol. NO scavenger cPITO and nitric oxide synthase (NOS)inhibitor PBITU prevent the latter two reactions, all of which are triggered in the absence of elicitor by NO donor sodium nitroprusside (SNP). The elicitor-induced NO release of Taxus chinensis suspension cells is strongly inhibited by PBITU. These results demonstrate a causal relationship between NO generation and the latter two reactions of Taxus chinensis cells to the elicitor, and also indicate that NO, produced via NOS in Taxus chinensis cells treated with fungal elicitor, might act as an essential signaling molecule for triggering the activation of PAL and synthesis of taxol.
作 者： Xu Maojun DONG Jufang ZHU Muyuan 作者单位： Xu Maojun(College of Life Sciences, Zhejiang University, Hangzhou 310012, China;College of Food Sciences and Biotechnology and Environmental Engineering, Hangzhou University of Commerce, Hangzhou 310035, China)DONG Jufang(College of Food Sciences and Biotechnology and Environmental Engineering, Hangzhou University of Commerce, Hangzhou 310035, China)
ZHU Muyuan(College of Life Sciences, Zhejiang University, Hangzhou 310012, China)
刊 名： 科学通报（英文版） SCI 英文刊名： CHINESE SCIENCE BULLETIN 年，卷(期)： 2004 49(10) 分类号： Q93 关键词： nitric oxide elicitor PAL Taxus chinensis cell taxol